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目的:建立一种比较系统,操作简单,成功率高的大鼠大脑中动脉缺血(MACO)再灌注动物模型,达到只要读者根据本文所述的方法操作就能制作出MACO再灌注模型的目的。方法:成年健康雄性SD大鼠40只,参照Longa法并适当改进建立MACO模型20只,假手术组20只。将详细叙述手术过程以及再灌注时间点的合理选择。最后利用行为学测试、四氮唑(TTC)染色对模型成功与否进行判定。结论:线栓法是一种操作简单的制备MACO再灌注动物模型的方法,并且此方法的再灌注效果较为明显。
Abstract：Objective:To establish a model of rat ischemia-reperfusion injury,in terms of the model,the operation will be simple,and the achievement ratio will be high.Methods:40 Male Sprague-Dawley(SD)rats were separated into two groups.Results:20 were model of rat ischemia-reperfusion injury based on Longa method,and the other 20 were sham-operated group.The process of the operation and the selection of different time point following ischemic-reperfusion were discussed in the paper.What's more,the model was appraised by behavioral test and Triphenyl Tetrazolium Choloride(TTC)Staining.Conclusion:The operation of intraluminal suture method is very simple for the establishment of model of rat ischemia-reperfusion,what's more,the effect of reperfusion is very obvious.
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基本信息:
DOI：10.13193/j.archtcm.2009.06.81.maxd.072
中图分类号:R-332
引用信息:
[1]马贤德,孙宏伟,柴纪严,等.线栓法制备大鼠脑缺血再灌注模型的方法研究[J].中华中医药学刊,2009,27(06):1200-1201.DOI:10.13193/j.archtcm.2009.06.81.maxd.072.
基金信息:
国家重点基础研究发展计划(973计划)资助项目(2007CB512707)
2009-06-10
2009-06-10