| 1,310 | 145 | 29 |
| 下载次数 | 被引频次 | 阅读次数 |
目的:建立一种比较系统,操作简单,成功率高的大鼠大脑中动脉缺血(MACO)再灌注动物模型,达到只要读者根据本文所述的方法操作就能制作出MACO再灌注模型的目的。方法:成年健康雄性SD大鼠40只,参照Longa法并适当改进建立MACO模型20只,假手术组20只。将详细叙述手术过程以及再灌注时间点的合理选择。最后利用行为学测试、四氮唑(TTC)染色对模型成功与否进行判定。结论:线栓法是一种操作简单的制备MACO再灌注动物模型的方法,并且此方法的再灌注效果较为明显。
Abstract：Objective:To establish a model of rat ischemia-reperfusion injury,in terms of the model,the operation will be simple,and the achievement ratio will be high.Methods:40 Male Sprague-Dawley(SD)rats were separated into two groups.Results:20 were model of rat ischemia-reperfusion injury based on Longa method,and the other 20 were sham-operated group.The process of the operation and the selection of different time point following ischemic-reperfusion were discussed in the paper.What's more,the model was appraised by behavioral test and Triphenyl Tetrazolium Choloride(TTC)Staining.Conclusion:The operation of intraluminal suture method is very simple for the establishment of model of rat ischemia-reperfusion,what's more,the effect of reperfusion is very obvious.
[1]Longa EZ,Weinstein PR,Carlson,et al.Reversible middle cere-bral artery occlusion without craniotomy in rat[J].Stroke,1989,20(1):84-91.
[2]Pulsinelli WA,Brierley JB.A new model of bilateral hemisphericexamination[J].Stroke,1979,10(3):267-272.
[3]冯新红,沈霞,袁伟,等.栓线法制作局灶性大鼠脑缺血再灌注模型的改进及效果[J].徐州医学院学报,2003,23(6):483-485.
[4]刘亢丁,苏志强,李毅平,等.实验性局灶性脑缺血再灌注动物模型的改进及评价[J].中风与神经疾病杂志,1997,14(2):87-89.
[5]王炳高,袁新颜,王守彪,等.线栓法制备大鼠脑缺血再灌注模型的改进[J].青岛大学医学院学报,2005,4(1):73-74.
[6]陈春富,李劲松.栓线法大鼠局灶性脑缺血模型的研究[J].中风与神经疾病杂志,1996,13(1):18-19.
[7]杨艳梅,冯晓源,崔梅,等.MRI对线栓法制作大鼠脑缺血再灌注模型分组的价值[J].临床放射学杂志,2007,26(9):917-920.
基本信息:
DOI：10.13193/j.archtcm.2009.06.81.maxd.072
中图分类号:R-332
引用信息:
[1]马贤德,孙宏伟,柴纪严,等.线栓法制备大鼠脑缺血再灌注模型的方法研究[J].中华中医药学刊,2009,27(06):1200-1201.DOI:10.13193/j.archtcm.2009.06.81.maxd.072.
基金信息:
国家重点基础研究发展计划(973计划)资助项目(2007CB512707)
2009-06-10
2009-06-10